Record the following DNA sequences of two individuals.

Person 1

G G | C C T C G G C C T A G A A C G G C C T A G C C G

C C | G G A G C C G G A T C T T G C C G G A T C G G C

Person 2

C T G A G G C C T A A G C G A T T C C C G G A T A T A

G A C T C C G G A T T C G C T A A G G G C C T A T A T

Digest the sequences using HaeIII. For each restriction site, draw a vertical line showing where the enzyme will cut. How many DNA fragments are produced for each individual following digestion with HaeIII?

Answer:

Answers

Answer 1

HaeIII is a restriction enzyme that recognizes the palindromic sequence "GG^CC" and cleaves the phosphodiester bond between G and the second C, generating a blunt-ended DNA fragment with a 4-base 5'-overhang.

The recognition site for the restriction enzyme HaeIII is "GG^CC", where the "^" represents the site of cleavage. The DNA sequences of person 1 and person 2 can be represented as:

Person 1:

GG|CCTCGGCC|TAGAACGGC|CTAGCCG

CC|GGA|GCCGGATCTTG|CCGGATCGGC

After digestion with HaeIII, the DNA fragments produced for person 1 are:

2 fragments of size 8 bp (cut at positions 3 and 14)

3 fragments of size 65 bp, 32 bp, and 16 bp (cut at positions 10, 21, and 28)

Person 2:

CTGAGGCC|TAAGCGATTC|CCCGGATATA

GACTCCGG|ATTCGCTAAG|GGGCCTATA

For person 2, the fragments produced are:

2 fragments of size 12 bp and 135 bp (cut at positions 7 and 19)

3 fragments of size 8 bp, 46 bp, and 22 bp (cut at positions 32, 38, and 45)

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