Decide if the following statements best describe map-based genome sequencing, best describe whole-genome shotgun sequencing, or apply equally to both sequencing methods.

a. starts with libraries of large, overlapping DNA fragments
b. starts cloning and sequencing of short, random DNA fragments
c. uses genetic recombination data to help arrange sequences correctly
d. requires sequences to be annotated after contig assembly
e. requires chromosome fragments to overlap for contig assembly
f. requires subcloning of large fragments into smaller clones for sequencing
g. is a better approach for repetitive sequences

1. Map-based genome sequencing
2. Whole-genome shotgun sequencing
3. Both sequencing methods

Answers

Answer 1

Answer:

1. Map-based genome sequencing: a; c; f; g

2. Whole-genome shotgun sequencing: b

3. Both sequencing methods: d; e

Explanation:

Map-based genome sequencing is a method that makes use of a reference genome sequence in order to determine the relative position of the DNA fragments before they are sequenced. This method is useful to determine the position of repetitive DNA fragments (for example, duplicated genes, repetitive non-coding regions, etc.) and Transposable Elements. Therefore, map-based genome sequencing is a suitable approach for large genomes (which are usually composed of repetitive sequences). On the other hand, in whole-genome shotgun sequencing, DNA sequences are obtained before the correct order of these DNA fragments is known. In this method, the genome is fragmented randomly into small DNA sequences (between 100 and 1000 base pairs), which are subsequently sequenced through the chain-termination sequencing approach (i.e., Sanger sequencing) and finally ordered by using bioinformatic tools that assemble overlapping reads.


Related Questions

describe the process of transformation from start to finish. Be sure to use the following terms in your response: restriction enzyme, ligase, plasmid, DNA, protein, gene, and sticky ends, and recombinant DNA.

Answers

The process of transformation are as follows:

restriction enzymes are used to cut the desired DNA from the organism as well the plasmid producing sticky endsThe plasmid is then inserted into the host organism.The host organism incorporates the plasmid producing recombinant DNA. The recombinant DNA is expressed by the host organism to produce proteins.

What is transformation?

Transformation is the process whereby genetic material from another organism is incorporated into the nucleus of another organism.

The steps in transformation are as follows:

restriction enzymes are used to cut the desired DNA from the organism as well the plasmid producing sticky endsThe plasmid is then inserted into the host organism.The host organism incorporates the plasmid producing recombinant DNA. The recombinant DNA is expressed by the host organism to produce proteins.

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(8.8A) Which star is hotter and brighter than our sun?

someone help me please

Answers

I would say maybe Betelgeuse than our sun but I’m not for sure so I’m sorry if you get it wrong have a good rest of your day/night :)

If one strand of DNA is A-C-G, then the other strand would be

Answers

The RNA would be U-G-C

which of these features is most likely formed as a result of the movement of earth plates in the diagram?

Answers

Answer:

A is the Answer

Explanation:

Who were the first guys to build a model of a DNA structure? (Thank you :). )

Answers

Answer:

James Watson and Francis Crick were mainly the first to build a model of a DNA structure. (Your welcome).

Explanation:

The long term survival of any species of organism is possible only if the organisms can

Answers

Adapt to their surrounding environment

Lab: Interdependence of Organisms
Assignment: Lab Report


Click the links to open the resources below. These resources will help you complete the assignment. Once you have created your file(s) and are ready to upload your assignment, click the Add Files button below and select each file from your desktop or network folder. Upload each file separately.

Your work will not be submitted to your teacher until you click Submit.

Answers

Answer:

Explanation:

what do you mean eh

Answer:

the dude up top is rigtht

Explanation:

Please help on both pls pls please please

Answers

Answer:

6. D   7. C

Explanation:

Answer:

6: D) Paramecium and animal cells are both a round shape.

7: C) Plant cells are square and animal cells are round.

Explanation:

6--

A) false. Animals are multicellular organisms

B) false. Paramecium and animal cells have round characteristics

C) false. Paramecium are unicellular organisms

D) true.

7--

A) false. Animal cells have round characteristics

B) false. Both plant and animal cells are multicellular organisms.

C) true. (the plant cell shape assists in the organisms' structure/resource movement)

D) false. Both plants and animals are made of cells, billions of them.

Ps.The answer is B,

William meets Kate They both share many of the same beliefs and interests. Based on the effects of similarity on
attraction, which of the following is most likely to be William's reaction?
A William will be more likely to trust Kate than he would a stranger
B. William will be more attracted to Kate than he would a stranger.
C. William will be more likely to love Kate than he would a stranger
D. William will be more likely to distrust Kate than he would a stranger
Please select the best answer from the choices provided
A

Answers

Answer  A

Explanation:

Answer:

the answer is B

Explanation:

just took the quiz

What is located between each vertebrae

Answers

Answer:

A Disc (Intervertebral)

Explanation:

Answer:

An internal disk is located between each vertebra. A gelatinous substance called nucleus pulposus can be found in each disk, which provides cushioning to the spinal column.

what must natural sciences educators pay attention to if they were to transform child science into true scientific understanding​

Answers

Integration of more practicals to demonstrate the power of science

3 chemical substances released by a plant into the environment ​

Answers

oxygen, carbon dioxide and your mom

What human behavior was the cause of the Nipah virus outbreak in Bangladesh? How did scientists determine this?

Answers

Answer:

Humans drinking raw date palm sap was the cause of the Nipah virus outbreak in Bangladesh. Scientists found Nipah virus in bat excretions and discovered that bats were contaminating date palm juice with their excretions (saliva, urine).

Explanation:

Nipah virus can spread to people from:

1) Direct contact with infected animals, such as bats or pigs, or their body fluids (such as blood, urine or saliva).

2) Consuming food products that have been contaminated by body fluids of infected animals (such as palm sap or fruit contaminated by an infected bat).

What family of KBOs does Orcus belong to?

Answers

Answer:

Pluto

Explanation:

Orcus is a plutino, a trans-Neptunian object that is locked in a 2:3 resonance with the ice giant Neptune, making two revolutions around the sun to every three of Neptunes. This is much as pluto, except that the phase of Orcus's orbit is opposite from Pluto's: Orcus is at aphelion when pluto is at aphelion when Pluto is at perihelion and vice versa. This was a major consideration in selecting its name, as deity Orcus was the Etruscan equivalent of the Roman Pluto, and later becamean alternate name for Pluto

Family of KBOs orcuas belongs to plutino, it is a plutino object revolves around the sun.

What is Family of  KBOs?

Orcus is a plutino, a trans-Neptunian object that revolves about the sun twice as often as Neptune and is locked in a 2:3 resonance with it.

This is quite similar to Pluto, but Orcus's orbital phase is the opposite of Pluto's, Orcus is at aphelion while Pluto is at perihelion, and vice versa.

This was a key factor in naming it since the deity Orcus was the Etruscan counterpart to the Roman Pluto and eventually developed into a different name for Pluto.

Therefore, revolves around the sun twice as often as Neptune is the plutino is a orcus in the family of KBOs.

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name the following for the phyla

Answers

Phylum Porifera (Sponges) ...
Phylum Coelenterata (Cnidaria) Corals & Jellyfish. ...
Phylum Ctenophora (Comb Jellies) ...
Phylum Platyhelminthes (Flatworms) ...
Phylum Nemertea (Ribbon Worms) ...
Phylum Rotifera (Rotifers) ...
Phylum Gastrotricha (Gastrotrichs) ...
Phylum Nematomorpha (Horsehair Worms)

phylum of wolf is Chordate , phylum of Earthworm is Annelid , phylum of sponge is Porifera, phylum of jellyfish is Cnidaria ,phylum of Sparrow is Chordate, Phylum of Butterfly is Arthropod.

which of the following is not one of the planes and sections of body orientation?

Answers

Answer: They are the median plane, sagittal planes, coronal (frontal) planes and horizontal (transverse) planes

Explanation:

Anatomical Planes in a Human: There are three primary planes in zoological anatomy: sagittal, coronal, and transverse. A human inside the anatomical function, can be described the usage of a coordinate system with the Z-axis going from the front to returned, the X-axis going from left to right, and the Y-axis going from up to down.

What are the three planes of movement inside the body?

Sagittal plane: Cuts the body into left and proper halves. ahead and backward actions.Coronal (or Frontal plane): Cuts the frame into front and back halves. facet-to-aspect actions.Transverse plane: Cuts the body into top and bottom halves. Twisting actions.

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the best way to define blastocyst

Answers

Answer:

A blastocyst is a fertilized egg that has developed for 5-6 days and contains 3 distinct features including a fluid-filled cavity trophectoderm/trophoblast (T) cells, and an inner cell mass (ICM). A blastocyst may also be referred to as a Day 5,6 or 7 embryo.

Explanation:

Answer:

A blastocyst is a fertilized egg that has developed for 5-6 days and contains 3 distinct features including a fluid-filled cavity trophectoderm/trophoblast (T) cells, and an inner cell mass (ICM). A blastocyst may also be referred to as a Day 5,6 or 7 embryo.

Which fact supports the following conclusion?

Public opinion caused the agriculture industry to rethink animal treatment.

O Organic food sales have been steadily rising since the 1990s.

O Livestock is often housed inside with regular periods of outdoor exercise.

O The demand for free-range and cage-free products has increased.

O Consumers are concerned about the health risks of genetic modification.

Answers

Answer:

hello i think b not completely sure

Explanation:

Answer:The demand for free-range and cage-free products has increased.

Explanation: Did it on Edge

Where is sugar made in plant cells?
O golgi apparatus
chloroplast
centriole
mitochondria

Answers

Answer:

the chloroplast

Explanation:

Sugar is made in the chloroplast of a plant cell. The chloroplast is an organelle that performs photosynthesis,

Answered by the ONE & ONLY #DRIPPQUEENMO

Why can king snakes eat rattlesnakes ?

Answers

Kingsnakes squeeze their prey to death, are immune to rattlesnake venom and are so named for their astonishing ability to overpower and eat snakes that are much larger than they are.

Describe the components of a buffer and the role each
plays in helping maintain a constant pH.

Answers

A buffer solution is made up of a weak acid and its conjugate base or a weak base and its conjugate acid. The two components maintain a pH balance that resists change when strong acids or bases are added to it.

The components of a buffer and the role each plays in helping maintain a constant pH are acid-base and its salt.

What is a buffer?

A weak acid and its conjugate base, or a weak base and its conjugate acid, make form a buffer solution. When strong acids or bases are added, the pH balance of the two components remains stable.

A buffer is a solution that withstands any pH change when a little amount of both an acid and a base is added to it. The salt of the acid or base must be present for a solution to qualify as a buffer.

For an acidic buffer, acid and its salt with a strong or weak base are necessary ingredients. As an illustration, consider an acetic acid and sodium acetate solution.

For a basic buffer, a base salt with a strong or weak acid is a necessary component.

Thus, the components of a buffer are acid, base, and salts.

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Which statement is true?

A. Temperatures on the Moon are always the same as the temperatures at the Earth’s poles.

B. Temperatures on the Moon are always hotter than the Earth.

C. Temperatures on the Moon are always colder than the Earth.

D. Temperatures on the Moon fall into a wider range than on the Earth.

Answers

D

Because the moon lacks an atmosphere and it’s day night cycle is so long, one side of the moon will spend a long time in the suns rays with no atmosphere to absorb the energy, while the other side will have no sunlight at all. The lit side will reach extremely high temperatures, and the dark side will reach extremely cold ones.

Many plants produce seeds which have small hooks on them. These hooks will attach to animals as they
move past the plants. What process do these hooks help the plant with?
A. pollination
B. fertilization
C. germination
D. seed dispersal

Answers

I believe the answer is D

Use the library and other reference materials to research lupus. Then, write a 400-word report on what you have learned. Be sure to include symptoms, causes, and treatments. Write your report in the essay box below.


if your answer is right you l get brainly

Answers

Answer:

Lupus refers to autoimmune disease in which the immune system of the body  attacks its normal and healthy tissue instead of virus and bacteria. Symptoms of this disease include inflammation, swelling, and damage to the joints, skin, kidneys, heart, and lungs etc. The main cause of this disease is the environment that triggers Lupus. Chemotherapy and medications such as Nonsteroidal anti-inflammatory drugs and Antimalarial drugs etc are used for its treatment. Due to the disease's complex nature, it is often called "the of 1000 faces". In the united states there are about 16000 new cases of lupus every year. There are different kinds of lupus. This article will focus mainly on systemic lupus erythematosus (SLE), but other types include discoid, drug-induced, and neonatal lupus. Currently there is no cure for lupus, but the symptoms can be managed with care. The treatment aims to manage and prevent flare ups. The exact treatment will depend on how lupus affects the individual. Without treatment, flares can occur that may have life-threatening consequences.

Explnation:

its not 400 words but it gives you an idea of what to do

Miss treatment or blank is defined as any action that inflicts pain suffering or injury

Answers

Mistreatment or ill-treatment is defined as any action that inflicts pain, suffering, or injury.

Do Birds Really Abandon Their Chicks If Humans Touch Them? 25 points!!

Answers

Answer:

no

Explanation:

"According to folklore, birds will reject their eggs and young if humans have so much as laid a finger on them. ... No matter how flighty birds appear, they do not readily abandon their young, especially not in response to human touch, says Frank B. Gill, former president of the American Ornithologists' Union."

define flower gardening​

Answers

Answer: A flower garden or floral garden is any garden where flowers are grown and displayed. ... Flower color is another important feature of both the herbaceous border and the mixed border that includes shrubs as well as herbaceous plants.

Explanation:

Answer:

the act whereby flowers are grown and displayed on a garden. it can also be called floral gardening.

Read each case file and fill in a cytogenetics report form for each case file.

Answers

Hi it won’t let me see the pic can you repost it plz for me to help :(())

Research It!
Part 2
SCIEnce
What is the difference between a dominant and
recessive allele? Give an example of each.

Answers

Answer:

the difference between dominate and recessive alleles are that the dominate allele will be expressed if it is present, while the recessive allele will only be expressed in the absence of the dominate trait

When thinking about genotypes and phenotypes, which is the cause and which is the effect?

Answers

Answer:

A particular genotype does not necessarily guarantee a particular phenotype. Understanding what factors can change this expression is quite complex.. What is what i believe that above statement meant.

Explanation:

Error is the stochastic variation associated with a particular observation of the phenotype (and is managed by use of replication and and appropriate experimental design). The phenotype is the result not only of the average effect of the different alleles at a given locus across test units and across environments, but is impacted by the interaction between the different alleles and the environments (including genetic backgrounds) in which they are evaluated. Phenotype is not simply the sum of the genotype effect and the environment effect, as indicated in the mathematical formula that you provide.

Hope I Helped!!!

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