Dull pain in the lower right abdomen that comes and goes can be caused by appendicitis, which is inflammation of the appendix.
Appendicitis occurs when the appendix becomes infected and fills with pus. The pain typically starts around the belly button and then moves to the lower right abdomen. If left untreated, the appendix can rupture and cause a serious infection in the abdomen.
Treatment usually involves surgery to remove the appendix. If you experience symptoms of appendicitis, such as severe abdominal pain, fever, nausea, vomiting, or loss of appetite, seek medical attention immediately. Early diagnosis and treatment are important to prevent complications.
Overall, Dull pain in the lower right abdomen that comes and goes can be caused by appendicitis, which is inflammation of the appendix.
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Record the following DNA sequences of two individuals.
Person 1
G G | C C T C G G C C T A G A A C G G C C T A G C C G
C C | G G A G C C G G A T C T T G C C G G A T C G G C
Person 2
C T G A G G C C T A A G C G A T T C C C G G A T A T A
G A C T C C G G A T T C G C T A A G G G C C T A T A T
Digest the sequences using HaeIII. For each restriction site, draw a vertical line showing where the enzyme will cut. How many DNA fragments are produced for each individual following digestion with HaeIII?
Answer:
HaeIII is a restriction enzyme that recognizes the palindromic sequence "GG^CC" and cleaves the phosphodiester bond between G and the second C, generating a blunt-ended DNA fragment with a 4-base 5'-overhang.
The recognition site for the restriction enzyme HaeIII is "GG^CC", where the "^" represents the site of cleavage. The DNA sequences of person 1 and person 2 can be represented as:
Person 1:
GG|CCTCGGCC|TAGAACGGC|CTAGCCG
CC|GGA|GCCGGATCTTG|CCGGATCGGC
After digestion with HaeIII, the DNA fragments produced for person 1 are:
2 fragments of size 8 bp (cut at positions 3 and 14)
3 fragments of size 65 bp, 32 bp, and 16 bp (cut at positions 10, 21, and 28)
Person 2:
CTGAGGCC|TAAGCGATTC|CCCGGATATA
GACTCCGG|ATTCGCTAAG|GGGCCTATA
For person 2, the fragments produced are:
2 fragments of size 12 bp and 135 bp (cut at positions 7 and 19)
3 fragments of size 8 bp, 46 bp, and 22 bp (cut at positions 32, 38, and 45)
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